Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4.5S RNA | stimulates GTPase activity in the SRP-FtsY complex in vitro and is essential for SRP function in vivo | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 4.5S RNA tetraloop mutants UUCG, UUUU, CUUC, GUAA and GAAA | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | the SRP targets the ribosome-nascent chain complexes to the inner membrane by interacting with the SRP receptor | Escherichia coli | 5829 | - |
inner membrane | the SRP targets the ribosome-nascent chain complexes to the inner membrane by interacting with the SRP receptor | Escherichia coli | - |
- |
ribosome | the SRP targets the ribosome-nascent chain complexes to the inner membrane by interacting with the SRP receptor | Escherichia coli | 5840 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Escherichia coli | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | SRP comprises the Ffh protein and 4.5S RNA, which together recognize hydrophobic signal peptides as they emerge from translating ribosomes | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Escherichia coli | GDP + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
heterodimer | Ffh and FtsY associate into a heterodimer upon GTP binding, upon GTP hydrolysis Ffh and FtsY dissociate | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
signal recognition particle | - |
Escherichia coli |
SRP | - |
Escherichia coli |
SRP GTPase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, UUUU mutant | Escherichia coli | |
0.037 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, no RNA | Escherichia coli | |
0.053 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, UUCG mutant | Escherichia coli | |
0.14 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, GUAA mutant | Escherichia coli | |
0.182 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, GAAA mutant | Escherichia coli | |
0.223 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, wild-type RNA | Escherichia coli | |
0.253 | - |
GTP | maximal rate constant for the GTPase activity of the SRP-FtsY complex, CUUC mutant | Escherichia coli |